Antioxidant And Enzyme Compounds


Antioxidant And Enzyme Inhibitory Constituents From Dactyladenia Floribunda Of Hennadiol

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Therefore investigations on specific other genera of this family resulted in the isolation and characterization of steroids, flavonoids, triterpenoids, and tannins. Like Parinari, Licania, and Chrysobalanus. You can say that this was the first phytochemical or pharmacological studies on any species of the genus Dactyladenia. Here we find the report on isolation and identification of known compounds like fatty acid, triterpenes, sugar, steroids, and flavonoids. The stems barks of the title plant studied for this report.

Antioxidant And Enzyme Inhibitory Compounds
Antioxidant And Enzyme Inhibitory Compounds

Moreover, chemical transformations were carried out on Hennadiol. It is one of the isolates compounds. The lipoxygenase, antioxidant, and urease inhibitory activities of the extract was determined. Along with these isolated and synthesized compounds were also included.

Process Of Compound Study

So, the crude extract (150.16 g) subjected to column chromatography over silica gel. Then eluted with hexane. Then followed by mixtures of hexane/EtOAc and EtOAc/MeOH in the increasing order of its polarity. Nine fractions were obtained (DF-I to DF-IX). The Fractions DF-I (70 g) subjected to CC over silica gel (300 g, column: 100 × 8 cm). Following which eluted with hexane then by hexane/ EtOAc (1:1).

This yielded six fractions DFI-a, DFI-b, DFI-c, DFI-d, DFI-e, DFI-f. It was resulting in six compounds of different weights. DFI- a gave Compound 1 of 20 mg. Compound 2 (200 mg) obtained from DFI-b. Similarly, DFI-c produced Compound 3 of 8 mg, and DFI-d gave compound 4 (15 mg). Compound 5 (40 mg) was from fraction DFI-e. Likewise, compound 6 (300 mg) [12] obtained from fraction DFI-f.

Furthermore, fraction DF-III (7.15 g) was subjected to column chromatography (CC) over silica gel (300 g, column: 80 × 4 cm) using hexane followed by hexane/EtOAc (6: 4) to furnish compound 7 (12 mg). Then compound 8 (17 mg) and compound 9 (100 mg), Compounds 10 (30 mg), 11 (50 mg) and 12 (64 mg) from fraction DF-VII (22 g). And after purification over CC using silica gel (200 g, column (50 × 4 cm) eluting with EtOAc/MeOH (9: 1).

Thereafter, the structures of these compounds established by their spectroscopic data and comparison with literature. Therefore treat pyridine solution of the compound (6) with acetic anhydride and then subjected to microwave treatment for sufficient time in 30-second intervals. Then, TLC analyzed small portions of the mixture every 30 seconds.

Antioxidant And Enzyme Inhibitory Constituents From Dactyladenia Floribunda Of Hennadiol Compound
Antioxidant And Enzyme Inhibitory Constituents From Dactyladenia Floribunda Of Hennadiol Compound

Results Of The Compounds Study

Then was conventional acetylation for the same type of reaction mixtures. Then heat it on a water-bath for an hour and keeping overnight. Hence, the results of TLC indicated the presence of small amounts of the unreacted compound. Hence, microwave-assisted deacetylation of hennadiol afforded the corresponding diacetyl derivative (13) (56 mg). While hydrogenolysis and subsequent separation of the resulting mixture by CC provided Lupeol (3) (17 mg), compound (14) (10 mg), and (15) (12 mg). Thus, all compounds obtained by chemical transformations identified by examination of the 1H, 13C, and 2D-NMR along with MS data. 

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